For the GFP fusion study, the TA images were captured from frozen sections by Nikon (Melville, NY) Eclipse E800 microscopy followed by hematoxylin-and-eosin staining of the same sections. The images were captured by a Deltavision SA3.1 wide-field deconvolution microscope (Applied Precision, Issaquah, WA). The myofibers were revealed by rhodamine-phalloidin staining (Molecular Probes). The sections were fixed in 4% paraformaldehyde and incubated with anti-human nuclear antibody (1:250) overnight at 4☌ followed by Alexa Fluor 488 goat anti-mouse IgG1 secondary antibody (Molecular Probes, Eugene, OR). The TA muscles were frozen in OCT embedding medium and sectioned as 6-μm longitudinal sections. For further details, including warnings about image enhancement, see “Important guidelines for image preparation“ on the Blood Manuscript Submission page. Insets are generally explained in the figure legend, unless their meaning is obvious from the image.įor each color photomicrograph in a figure, the stain used should be specified in the legend.įor each micrograph, the following information must be provided, either in the methods section or in the figure legend: (1) microscope’s make and model, (2) type, magnification, and numerical aperture of the objective lenses, (3) temperature, (4) imaging medium, (5) fluorochromes, (6) camera’s make and model, (7) acquisition software, and (8) any subsequent software used for image processing. In that case, the key should be placed into the figure image as compactly as possible. If necessary, symbols may be explained in a key within the figure image. Splenocytes from DO11.10 mice were incubated with 0, 1, 10, 100, or 1000 ng of MIP-1a per mL (triangles) or MIP-1b (squares) in plates containing 0 (open symbols) or 500 (filled symbols) μg of OVA per ml. If necessary, words may be used to describe the symbols. Symbols represent IVIG treatment groups (n = 4 rats/group): saline ( Symbols should be explained in the text legend if possible, including the symbols themselves if possible. (C) Plasma levels from 10 healthy control (Ctrl) donors.Īny abbreviations used in the figure image but not explained in the article's main text should be defined in the legend. In the example below, “control” should be abbreviated “Ctrl,” not “C,” to avoid confusion with panel C of the figure. Avoid any text labels that might be confused with panel and subpanel labels. (D) Representative genes that are highly expressed in CMPs.įigure images may contain symbol and text labels that are necessary to convey relevant detail. (C) Representative genes that are highly expressed in CLPs. (B) Representative genes that were upregulated in MPPs. (A) Representative genes that are predominantly expressed in HSCs and downregulated in MPPs, CLPs, and CMPs. The vertical axis represents the normalized gene expression values. Clusters of genes categorized by the expression patterns in purified stem and progenitor cells. A nonboldface description of the figure usually follows, run in after the title, describing each panel, subpanel, inset, or other part of the figure.įigure 4. Do not refer to figure panels, other figure parts, or any other part of an article in a figure title. The figure legend must contain a boldface (a) name ("Figure" + arabic figure number) and (b) substantive title. However, if the tabular material is graphically related to parts of a figure, it can appear within the figure. Tabular material should almost always appear as a table that is not part of a figure. The maximum width is 17.7 cm (2-column width). In order to prevent radical changes in figure content, authors should prepare the figures 8.4 cm wide (1-column width) or, if necessary, 12.7 cm wide (1½ column width). If an article is accepted for publication, the figures may be altered by Blood's publication management vendor to conform to Blood style, which includes, but is not limited to, standard colors and fonts. Images will be sized to fit the smallest possible space while retaining all relevant detail. Images should be laid out as compactly as is consistent with conveying the relevant data.
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